FIP & MITP Seminar: Pushing the boundaries of multiphoton brain imaging
Multiphoton microscopy has changed how we visualize neurons by providing high-resolution, non-invasive imaging capability deep within intact brain tissue. Multiphoton imaging will likely play a major role in understanding how the brain works at the level of neural circuits. In this talk, the fundamental challenges of deep tissue, high-resolution optical imaging are discussed. New technologies for in vivo structural and functional imaging of mouse brain using long wavelength excitation and three-photon microscopy will be presented. We will illustrate the requirements for imaging the dynamic neuronal activity at the cellular level over a large area and depth in awake and behaving animals, and show applications where 3-photon microscopy outperforms conventional 2-photon microscopy in both signal strength and image contrast. Finally, we will discuss several future directions, including adaptive optics and new laser sources, to further improve the imaging depth and speed in biological tissues.
Chris Xu is Professor of Applied and Engineering Physics, Cornell University. He is the founding co-director of Cornell Neurotech, and the director of Cornell NeuroNex Hub, an NSF funded center for developing and disseminating neurotechnology. Prior to Cornell, he was a member of technical staff at Bell Laboratories, and pioneered breakthrough development of fiber optic communication systems. He received his Ph.D. in Applied Physics from Cornell University.