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BEGIN:VEVENT

CATEGORIES:Medicine
CATEGORIES:Engineering
CATEGORIES:Natural Sciences
CATEGORIES:Lectures/Conferences
CATEGORIES:Utilities
CATEGORIES:Lecture/Talk
CATEGORIES:Panel/Seminar/Colloquium
CATEGORIES:Research
CATEGORIES:Main
CONTACT;X-BEDEWORK-UID=8a0183a7-83184018-0184-f88b4dd1-00001a24:Franklin\,
  Monica
CREATED:20230805T023014Z
DESCRIPTION:Biomolecular condensates are membrane-free compartments that s
 patially and temporally organize biochemistry in cells. Nucleic acids are
  frequence drivers and architectural elements in condensates however the 
 molecular grammar that dictates the composition and physical states of co
 ndensates remains mysterious.  To examine how RNA sequence and structure 
 impact the form and function of condensates\, we created an evolutionary 
 algorithm that designs shuffled mRNA sequences which maximize or minimize
  predicted free energies of folding while preserving mass\, known protein
  binding sites\, encoded amino acid sequence\, and nucleotide composition
 . RNAs with minimized free energies of folding contain many stable duplex
 es\, while RNAs with maximized energies have unstable structures and long
  single-stranded regions. Using mass photometry\, we show that RNAs with 
 stable structures are mostly monomeric\, while RNAs with unstable structu
 res can multimerize. When mixed with protein\, these differential RNA-RNA
  interactions affect dense phase compositions. Specifically\, unstable RN
 As form condensates with high RNA and low protein concentrations\, while 
 the converse is true for RNAs with stable structures. Using microbead rhe
 ology\, we observe that unstable RNAs form condensates with long viscoela
 stic relaxation times that increase with condensate age\, indicating elas
 tic properties at many timescales. By contrast\, stable RNAs form less el
 astic condensates with shorter relaxation times that increase to a lesser
  degree with time. We hypothesize that such aging is controlled by the ex
 change of intra- for inter-molecular base pairing among RNAs whose foldin
 g free energies determine the timescale of such exchanges. Thus\, RNA str
 ucture can have multi-scale consequences for biomolecular condensates\, w
 ith impacts on condensate composition and material properties.
DURATION:PT1H
DTSTAMP:20231031T183707Z
DTSTART;TZID=America/New_York:20231113T120000
LAST-MODIFIED:20231031T183707Z
LOCATION;X-BEDEWORK-UID=18832edc-1c2ecfef-011c-481f44ec-0000030a:French Fa
 mily Science Center 4233
STATUS:CONFIRMED
SUMMARY:Encoding Physical Properties of the Cytosol
UID:CAL-8a018d0d-8b5366bc-018b-87059ba3-000020e5demobedework@mysite.edu
X-BEDEWORK-ALIAS;X-BEDEWORK-PARAM-DISPLAYNAME=Main:/user/public-user/Utili
 ties/Main
X-BEDEWORK-ALIAS;X-BEDEWORK-PARAM-DISPLAYNAME=Engineering:/user/public-use
 r/Topics/Engineering
X-BEDEWORK-ALIAS;X-BEDEWORK-PARAM-DISPLAYNAME=Medicine:/user/public-user/T
 opics/Medicine
X-BEDEWORK-ALIAS;X-BEDEWORK-PARAM-DISPLAYNAME=Natural Sciences:/user/publi
 c-user/Topics/Natural Sciences
X-BEDEWORK-ALIAS;X-BEDEWORK-PARAM-DISPLAYNAME=Research:/user/public-user/T
 opics/Research
X-BEDEWORK-ALIAS;X-BEDEWORK-PARAM-DISPLAYNAME=Lecture_Talk:/user/public-us
 er/Lectures_Conferences/Lecture_Talk
X-BEDEWORK-ALIAS;X-BEDEWORK-PARAM-DISPLAYNAME=Panel_Seminar_Colloquium:/us
 er/public-user/Lectures_Conferences/Panel_Seminar_Colloquium
X-BEDEWORK-CS;X-BEDEWORK-PARAM-DESCRIPTION="/principals/users/agrp_Schoolo
 fMedicine_BiostatisticsandBioinformatics,/principals/users/agrp_Centerfor
 GenomicandComputationalBiology,/principals/users/agrp_AGPM_Collaboratory,
 /principals/users/agrp_SchoolofMedicine,":Biostatistics and Bioinformatic
 s\,Duke Center for Genomic and Computational Biology (GCB)\,Precision Gen
 omics Collaboratory\,School of Medicine (SOM)
X-BEDEWORK-SPEAKER:Amy Gladfelter
X-BEDEWORK-DUKE-SERIES:CBB Monday Seminar Series
X-BEDEWORK-IMAGE-X1:0
X-BEDEWORK-IMAGE-Y1:0
X-BEDEWORK-IMAGE-X2:529.5
X-BEDEWORK-IMAGE-Y2:353
X-BEDEWORK-IMAGE-CROP-WIDTH:529.5
X-BEDEWORK-IMAGE-CROP-HEIGHT:353
X-BEDEWORK-IMAGE-ALT-TEXT:Amy Gladfelter PhD\, Professor\, Cell Biology Du
 ke University
X-BEDEWORK-SUBMITTEDBY:monicaf for Computational Biology and Bioinformatic
 s (CBB) (agrp_SchoolofMedicine_CBB)
X-BEDEWORK-IMAGE:/public/Images/Amy Gladfelter canva_20231031063613PM.png
X-BEDEWORK-THUMB-IMAGE:/public/Images/Amy Gladfelter canva_20231031063613P
 M-thumb.png
END:VEVENT
END:VCALENDAR