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BEGIN:VEVENT

CATEGORIES:Lectures/Conferences
CATEGORIES:Utilities
CATEGORIES:Lecture/Talk
CATEGORIES:Panel/Seminar/Colloquium
CATEGORIES:Main
CONTACT;X-BEDEWORK-UID=18832e99-20476e5b-0120-587daa86-000000bc:Burns\, Au
 gust
CREATED:20240826T170936Z
DESCRIPTION:Recording large-scale brain activity patterns at cellular reso
 lution in freely-behaving mice using current recording approaches present
 s several challenges. First\, linear microscopy methods lack the depth pe
 netration and optical sectioning required for deep-tissue recording\, whi
 le multiphoton microscopy allows for recording neuronal activity only fro
 m a single plane or few axially-shifted planes. In addition\, cellular-re
 solution recording requires a mouse to either be head-fixed under a micro
 scope or to have a miniaturized imaging device attached onto its skull. B
 oth can impact mouse behavior and underlying brain activity. Recently\, m
 y lab showed that utilizing an integrator for calcium-dependent recording
  of neuronal activity (CaMPARI) allows for cellular-resolution imaging of
  large-scale brain activity patterns in freely-behaving mice without need
 ing to attach any mechanical device. It was also shown that despite the e
 nhanced in vitro performance of the second generation of CaMPARI (CaMPARI
 2)\, it exhibits poorer in vivo neuronal activity recording sensitivity w
 hen compared to CaMPARI1 in mice. Here\, we present an ongoing work to de
 velop a new generation of the CaMPARI sensor (CaMPARI3)\, which will over
 come the limitations of the previous generations. We developed an in vitr
 o-in vivo screening pipeline and found that the photoconversion propertie
 s of the same sensors in vitro and in vivo do not correlate. Rather\, the
  peak DF/F in vitro may better predict the rate of photoconversion in viv
 o. We present our progress with developing CaMPARI3\, which exhibits enha
 nced photoconversion and also the ability for recording dynamic changes i
 n neuronal activity. Therefore\, CaMPARI3 is expected to expand the possi
 ble experimental applications to record single-cell resolution brain acti
 vity from freely-behaving mice.\nHod Dana received his B.Sc. (summa cum l
 aude) and Ph.D. degrees in Biomedical Engineering from the Technion - Isr
 ael Institute of Technology. During his PhD work he developed advance non
 linear microscopy methods for recording of large-scale neuronal activity.
  Dr. Dana did his post-doctoral training with the GENIE project at the Ho
 ward Hughes Medical Institute Janelia Research Campus. As a postdoctoral 
 researcher\, he was involved in the development and testing of new calciu
 m sensors for recording neuronal activity.
DURATION:PT1H
DTSTAMP:20240826T170936Z
DTSTART;TZID=America/New_York:20241016T120000
LAST-MODIFIED:20240826T170936Z
LOCATION;X-BEDEWORK-UID=18832e99-20476e5b-0120-58864347-000000be:Fitzpatri
 ck Center Schiciano Auditorium Side A\, room 1464
STATUS:CONFIRMED
SUMMARY:FIP Seminar: Development and application of a calcium sensor for r
 ecording neuronal activity in freely-moving mice at cellular resolution
UID:CAL-8a00048d-91324965-0191-8faa202f-00007c61demobedework@mysite.edu
X-BEDEWORK-ALIAS;X-BEDEWORK-PARAM-DISPLAYNAME=Main:/user/public-user/Utili
 ties/Main
X-BEDEWORK-ALIAS;X-BEDEWORK-PARAM-DISPLAYNAME=Lecture_Talk:/user/public-us
 er/Lectures_Conferences/Lecture_Talk
X-BEDEWORK-ALIAS;X-BEDEWORK-PARAM-DISPLAYNAME=Panel_Seminar_Colloquium:/us
 er/public-user/Lectures_Conferences/Panel_Seminar_Colloquium
X-BEDEWORK-SPEAKER:Dr. Hod Dana\, Associate Staff\, Department of Neurosci
 ences\,  Lerner Research Institute\, Cleveland Clinic Foundation  Assista
 nt Professor of Molecular Medicine\,  Cleveland Clinic Lerner College of 
 Medicine\, Case Western Reserve University
X-BEDEWORK-SUBMITTEDBY:ahb11 for Fitzpatrick Institute for Photonics (FIP)
  (agrp_FitzpatrickInstitute)
END:VEVENT
END:VCALENDAR