Microscopy and TSA-Seq Mapping of Nuclear Genome Organization Point to Nuclear Speckles as a Major Transcriptional Hub

My primary research interests as an independent investigator have been to understand the highest levels of chromatin and chromosome folding, which we have termed large-scale chromatin structure. We also have found that different large-scale chromatin compaction states are
tightly correlated with differential positioning within the nucleus, and surprisingly in certain cases we have observed directed, long-range interphase chromosome movements coupled to transcriptional activation. Research in my laboratory is now increasingly focusing on the functional impact this large-scale chromatin organization has on DNA functions such as transcription and replication.