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FIP Seminar: Fourier Light Field Imaging for High Speed, 3D Analysis in the Mesoscale Regime

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Wednesday, March 05, 2025
12:00 pm - 1:00 pm
Clare Cook, BME PhD Candidate, Duke University

In this work, we address this challenge by adapting Fourier Light Field Microscopy [3] (an established method for high-speed, 3D microscopy) to the mesoscale regime alongside computational imaging techniques. Specifically, we present two novel imaging systems which work in distinct application spaces. First, our Fourier Light Field Multiview Stereoscope (FiLM-Scope) consists of a high-SBP primary lens placed in front of a multi-camera array microscope (MCAM) [4] to acquire 48 high resolution, multi-perspective images of a 3 cm x 3 cm field-of-view. Alongside a self-supervised machine learning algorithm, this system can provide nearly isotropic resolution in all three dimensions down to 20 um to improve 3D visualization in microsurgical settings. The second system is our Ultra-high Speed Fourier Light Field Mesoscope (US-FLFM), which combines Fourier Light Field Imaging with an ultra-high speed camera to provide multi-perspective videos of a ~3x3mm area at over 100kHz frame rates. We are currently using this system to study energy flow in the exoskeletons of snapping trap jaw ants.
Taken together, these systems demonstrate how Fourier Light Field imaging at the mesoscale can transform how we study systems-level biology.

Contact: August Burns