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Applications for In Vitro High-Throughput Profiling Assays In Next Generation Chemical Risk Assessment

Seminar speaker, Dr. Joshua Harrill, standing in front of US EPA flag.
Thursday, February 08, 2024
12:00 pm - 1:15 pm
Joshua Harrill, PhD; Cellular and Molecular Toxicologist, US EPA
Integrated Toxicology & Environmental Health Seminar Series

The Blueprint for Computational Toxicology at the US EPA proposes the use of high-throughput profiling assays in human-derived in vitro models as the first tier in chemical hazard evaluation. To date, two assays have been identified for use in this first tier of testing: high-throughput transcriptomics (HTTr) with the TempO-Seq assay which evaluates changes in gene expression in response to chemicals and high-throughput phenotypic profiling (HTPP) with the Cell Painting assay which evaluates changes in cell morphology in response to chemicals. Both of these assays can be deployed across many different human-derived in vitro models in order to evaluate different aspects of human biology, provide high content data that can be used to identify benchmark concentrations for biological activity, group chemicals with similar bioactivity profiles and inform putative mechanism-of-action predictions. This presentation will discuss each of these concepts and potential applications of HTTr and HTPP in next generation chemical risk assessment.

THIS IS A HYBRID SEMINAR WITH IN-PERSON & REMOTE ATTENDANCE OPTIONS.
This seminar will be held in Field Auditorium (room 1112), Grainger Hall.
Visit the seminar website for a livestream link to tune in virtually.
Both attendance options are free and open to all.